2007 Annual Research Course in Flow Cytometry

 

Los Alamos, New Mexico, June 9-15, 2007

Sponsored by: The National Flow Cytometry Resource (NFCR) and Verity Software House

 

Thank you to everyone that attended the 30th Annual Course in Flow Cytometry. We had a great time!

 

Please view the Photos from the course

CV data from Build a Flow Cytometer lab

 

Dear FLOWers:

The National Flow Cytometry Resource at the Los Alamos National Laboratory will be hosting the 30th Annual Research Course in Flow Cytometry.  Detailed information on the course content is provided below.  The course fee of $1,700 includes a welcoming reception on Saturday evening, lunches during the week, two evening sessions with refreshments and a banquet on Thursday evening.  Complete information is available on the NFCR home page at: http://nfcr.lanl.gov/.  More information and application materials can be obtained from Ms. Ruby Archuleta, National Flow Cytometry Resource, Mail Stop M888, Los Alamos National Laboratory, Los Alamos, NM 87545 [voice: (505)667-3912, FAX: (505)665-4096, email: rubyra@lanl.gov]. Please contact Ruby Archuleta prior to sending registration for the course.

 

Jim Freyer

 

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Course Description:  A methods course in flow cytometry will be offered with special emphasis on applications in cell biology, immunology and screening.  The faculty consists of distinguished members of the flow cytometry community.  This course will be aimed toward individuals with several years of experience in flow cytometry; very little basic material will be presented.  Each participant will receive extensive lecture notes and laboratory hand-out materials.  Course lecturers and their presentation subjects are listed below.  In addition, there will be two informal evening sessions, one on Biosafety/Core Operator issues and one on state-of-the-art flow instrumentation at the NFCR.  Finally, there will be a Special Lecture on applications of flow cytometry to analytical chemistry by Dr. Norman Dovichi of the Department of Chemistry, University of Washington.

                                               

#	Lecturer	Subject
1	John Martin	Basics of Flow Cytometry
2	Alan Waggoner	Fluorescent Probes & Fluorescence Detection
3	Alice Givan	DNA Analysis
4	Bruce Bagwell	The ABC’s of Software Compensation
5	Vincent Shankey	Analysis of Intracellular Epitopes
6	William Telford	Apoptosis
7	Scott Cram	Chromosome Analysis, Sorting, and Genomics
8	Joseph Trotter	Sorting
9	Carlton Stewart	Strategies
10	David Galbraith	Fluorescent Proteins in Flow & Imaging
11	John Nolan	Multiplexed and Microparticle-based Analysis
12	Charles Goolsby	Flow in Patient Management
13	Gerhard Nebe-von-Caron	Microbiology: Measuring bugs and other small bits
14	Larry Sklar	High Throughput Screening for Drug Discovery
15	Steven Graves	New Developments in Flow Cytometry Instrumentation

   
                                                                                                                                   

 

Laboratory Sessions will address sample preparation, instrument construction, data collection and analysis, common and more cutting edge flow assays, and typical problems encountered in flow cytometry.  Lab sessions will be hands-on, with plenty of time and opportunity for interaction with the instructors.  Lab sessions will utilize a variety of commercial and developmental instruments, and representatives from several of the commercial vendors will be available to demonstrate and discuss their most recent instruments and techniques.  Each participant will be given the choice of attending six laboratory sessions chosen from the topics below:

 

#	Instructor	Subject
1	Martin/Wilder/Jett	Build a Flow Cytometer
2	Bagwell	Compensation: From 'Nuts and Bolts' to the Sublime
3	Givan	Analysis of the DNA Content of Cells by Single-Color Flow Cytometry
4	Stewart/Stewart	Immunophenotyping
5	Goolsby/Shankey	Multiparameter Cell Cycle
6	Telford	Apoptosis
7	Nolan	Calibration and Standardization in Flow Cytometry
8	Trotter	Sorting
9	Galbraith/Hawley	Fluorescent Protein Analysis
10	Cram	Chromosome Analysis
11	Edwards	High Throughput Screening
12	Nebe-von-Caron	Microbiology

                                                                                                                                  

 

For more information please see the 1st letter to participants

 

To register, fill out the application and contact Ruby Archuleta [voice: (505)667-3912, FAX: (505)665-4096, email: rubyra@lanl.gov].

 

Maps and Driving Directions to Los Alamos

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The 28th Annual Course in Flow Cytometry:

Photos from the course and CV data for the Build-a-Flow Cytometer Labs