Lab 9 - Fluorescent Protein Analysis

David Galbraith and Teresa Hawley

 

Fluorescent Proteins comprise a family of related reporter molecules that can be conveniently expressed and detected within living cells and organisms.  This laboratory is designed to illustrate the use of four different Fluorescent Proteins, distinguishable according to excitation and emission spectra, for labeling mammalian cell lines, and their detection and separation using flow cytometry and cell sorting. Due to overlapping excitation spectra, multiple fluorescent proteins (FPs) can be excited by a single excitation wavelength, with as few as two laser lines are sufficient to excite four different FPs.