Fluorescent protein analysis and sorting

 

David Galbraith (University of Arizona), Georgina Lambert (University of Arizona), Teresa Hawley (George Washington University Medical Center)

 

Instrument: Becton Dickenson Influx

Fluorescent Proteins (FPs) comprise a family of related reporter molecules that can be conveniently expressed and detected within living cells and organisms. This laboratory is designed to illustrate the simultaneous detection of five spectrally compatible FPs (ECFP, EGFP, EYFP, DsRed and HcRed). Sp2/0 mouse hybridoma cells stably expressing the individual or multiple FPs have been generated for this purpose. FACS isolation of various populations will be performed by course participants. Simultaneous evaluation of EGFP and DNA content will also be performed using plant tissues.