Steve Graves
Contact Info
graves@lanl.gov
505 667 4652
Profile
The National Flow Cytometry Resource – This activity centers around the development of new flow cytometry assays and instrumentation for biomedical and biotechnology applications. The NFCR has numerous flow cytometers and flow sorters to aid in the current application. The general goal of this project is technology development. I am one of four research PI’s on this project and as such have full access to all of the NFCR’s resources (NIH 5P41RR001315-25, Freyer - PI).
Low Cost Portable Flow Cytometry – This activity is geared to developing a low cost portable flow cytometer for use in resource poor settings. In this project we are developing all aspects of a miniature low cost portable flow cytomter. Technologies developed here have and are serving as seeds for spin-off diagnostics and will provide new technologies for many analysis applications. I am the PI of this project. (NIH 5R21RR020064-03, Graves – PI).
Rapid Iterative Detection of Massive Numbers of Pathogens – This activity is developing field based particle manipulation assays, signatures and instrumentation for the automated detection of large numbers of pathogens. This project will also develop ancillary diagnostic technologies and assays of value to almost any biotechnology project. I am PI on this project (DOE LANL LDRD DR20070010).
High-Throughput Flow Cytometry and Protease Screening – This activity is geared towards the development of high throughput flow cytometry assays and instrumentation specifically for protease activity. It will have synergy with any assay or assay target requiring high throughput screening. I am co-PI on this project with Dr. Bruce Edwards of UNM. (UNM Joint Science and Technology Laboratory funding).
Raman Spectral Flow Cytometry – In this activity we are developing new instrumentation, reagents and assays for a novel flow cytometry instrument designed to measure the Raman signature of individual particles. Porject will be synergistic with any project requiring high information content screening. I am co-PI on this project. (DOE LANL LDRD ER funding. Doorn – PI).
Recent Publications
Graves SW, Nolan JP, Sklar LA (2005) Molecular assemblies, probes
and proteomics in flow cytometry. In: Flow Cytometry in Biotechnology,
Sklar LA, ed. (Oxford University Press, London) Chapter 9: 153-177.
Graves SW, Woods TA, Kim H, Nolan JP (2005) Direct fluorescent staining
and analysis of proteins on microspheres using CBQCA. Cytometry A 65:
50-58.